Lipid contents in sinking diatom aggregates incubated in rotating pressure and control tanks


The effect of increasing hydrostatic pressure on the microbial degradation, the organic matter composition, and the microbiome of 'marine snow' particles was studied in laboratory incubation experiments. Model aggregates were produced from the diatom Skeletonema marinoi and the natural microbial community of surface seawater collected in the Kattegat. The aggregates were incubated individually in rotating pressure and control tanks to keep them suspended during 20-day incubations in the dark and at 3°C. In the pressure tanks, hydrostatic pressure was increased at increments of 5 MPa per day to finally reach 100 MPa. This pressure scheme simulates the descent of diatom aggregates from the surface ocean down into a 10-km deep hadal trench. In the control tanks, pressure was always left at atmospheric level.Volumetric contents of diatom-specific lipids in sinking diatom aggregates were determined by High Performance Liquid Chromatography (HPLC) coupled to quadrupole Time-of-Flight Mass Spectrometry (qToF-MS) in samples retrieved every 4 days throughout the 20-day incubation experiment.

Related Identifier
Metadata Access
Creator Becker, Kevin W ORCID logo; Elvert, Marcus ORCID logo
Publisher PANGAEA
Publication Year 2023
Funding Reference Danish National Research Foundation Crossref Funder ID DNRF145 Danish Center for Hadal Research, HADAL
Rights Data access is restricted (moratorium, sensitive data, license constraints)
OpenAccess false
Language English
Resource Type Dataset
Format text/tab-separated-values
Size 204 data points
Discipline Earth System Research
Spatial Coverage (10.427 LON, 55.368 LAT)
Temporal Coverage Begin 2021-07-27T13:48:26Z
Temporal Coverage End 2022-11-02T08:27:14Z