Floating Lipid Bilayers Created by Vesicle Fusion

DOI

'Floating LSupported Bilayers' (FSB's) have become the 'standard' systems used for studying membrane systems using reflectivity at ISIS and other facilities. They are reliable, reproducable models for biomembrane experiments, and can be fabricated from a variety of lipid compositions. One remaining unknown however, is how to introduce integral membrane proteins. It has long been recognised that one potential method would be to reconstitute MP's into vesicles, and then form the bilayers from these using vesicle fusion. However, up to now, all attempts to produce such membranes by vesicle fusion have been unsuccessful. Recently, we have finally demonstrated the fabrication of FSB's using vesicle fusion onto thiolipid SAM's bound to gold. This experiment is aimed at building on this preliminary data, to understand the factors affecting bilayer formation in this way.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24091317
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24091317
Provenance
Creator Dr Stephen Roser; Miss Emma Daulton; Dr Arwel Hughes; Professor Jeremy Lakey; Dr Stephen Holt
Publisher ISIS Neutron and Muon Source
Publication Year 2016
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2013-07-28T23:00:00Z
Temporal Coverage End 2013-08-04T21:34:47Z