Temperature effects on a plankton community from Helgoland Roads tested in an indoor mesocosm experiment in March 2022


To investigate the effect of temperature on a North Sea spring bloom community, we performed an incubation experiment in the mesocosm facility of the Institute for Chemistry and Biology of the Marine Environment (ICBM) in Wilhelmshaven. The plankton community was sampled from the long-term ecological research station Helgoland Roads (https://deims.org/1e96ef9b-0915-4661-849f-b3a72f5aa9b1) on the 6ᵗʰ of March, 2022. Collection of the surface community was conducted from the RV Heincke with a pipe covered with a 200 µm net that was attached to a diaphragm pump. The month-long incubation was started on the 7ᵗʰ of March in twelve indoor mesocosms, the Planktotrons (Gall et al., 2017). We chose three temperatures along the ascending part of the thermal performance curve (TPC) of the in situ community: the minimum temperature for positive growth (6°C, also the field temperature), the middle between the minimum and the optimum temperature (12 °C), and the optimum temperature for growth (18 °C). Ramping up the temperatures was conducted by 1 °C per day until the treatment temperatures were reached, resulting in a ramp phase (first twelve days) and a constant temperature phase. This dataset comprises all data collected within the experiment. Temperature, oxygen, pH, salinity, and in vivo fluorescence were measured daily at 10 am. Samples for dissolved nutrients (nitrate, nitrite, phosphate, silicate), chlorophyll a, DNA, particulate nutrients (biogenic silica, particulate organic carbon/nitrogen/phosphorus), as well as flow cytometric counts of bacteria (stained) and the unstained community were sampled every third day at the same time. The mesocosm water was generally filtered over a 200 µm mesh before sampling to exclude mesozooplankton. However, due to the appearance of large Phaeocystis colonies, additional samples without pre-filtration were taken for particulate organic carbon, nitrogen, phosphorus, and chlorophyll a starting on incubation day 15. PAR, total nitrogen and phosphorus as well as total alkalinity were measured at the start, in the middle, and at the end of the incubation. Samples for Mesozooplankton enumeration were taken and plankton species identified at the end of the experiment. All analysis scripts can be found on github (https://github.com/AntoniaAhme/TopTrons22MesocosmIncubation). The sequence data are available at the European Nucleotide Archive (ENA).

For more information about the research site Helgoland Roads visit https://deims.org/1e96ef9b-0915-4661-849f-b3a72f5aa9b1

DOI https://doi.org/10.1594/PANGAEA.961155
Related Identifier References https://doi.org/10.1594/PANGAEA.961508
Related Identifier References https://doi.org/10.1016/0198-0149(87)90021-5
Related Identifier References https://doi.org/10.1002/lom3.10196
Related Identifier References https://doi.org/10.4319/lo.1973.18.6.0897
Related Identifier References https://doi.org/10.1371/journal.pone.0137645
Related Identifier References https://github.com/AntoniaAhme/TopTronsMesocosmIncubation
Related Identifier References https://doi.org/10.3334/CDIAC/OTG.CO2SYS_XLS_CDIAC105A
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.961155
Creator Ahme, Antonia (ORCID: 0000-0003-1201-009X); Happe, Anika ORCID logo; Striebel, Maren ORCID logo; Olsson, Markus; Kühne, Nancy ORCID logo; Giesler, Jakob; Sentimenti, Alexander; Schulte-Hillen, Ruben L; Cabrerizo, Marco J ORCID logo; Kline, Simon; Neun, Sebastian; Naus-Wiezer, Suzanne M; Rickels, Heike; Van de Waal, Dedmer B ORCID logo; Torres-Valdés, Sinhué ORCID logo; John, Uwe ORCID logo
Publisher PANGAEA
Publication Year 2023
Funding Reference Helmholtz Association of German Research Centres https://doi.org/10.13039/501100001656 Crossref Funder ID Changing-Earth_Subtopic_6.2 https://earthenvironment.helmholtz.de/changing-earth/topics/topic-6-marine-and-polar-life/#c115556 Adaptation of marine life: From genes to ecosystems; Horizon 2020 https://doi.org/10.13039/501100007601 Crossref Funder ID 871081 https://cordis.europa.eu/project/id/871081 AQUACOSM-plus: Network of Leading Ecosystem Scale Experimental AQUAtic MesoCOSM Facilities Connecting Rivers, Lakes, Estuaries and Oceans in Europe and beyond
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Resource Type Dataset
Format text/tab-separated-values
Size 8288 data points
Discipline Chemistry; Computer Science, Electrical and System Engineering; Design; Engineering Sciences; Fine Arts, Music, Theatre and Media Studies; Humanities; Microsystems; Natural Sciences; Systems Engineering
Spatial Coverage (7.900 LON, 54.183 LAT); North Sea