The structure and orientation of the Bam complex in the Gram-negative outer membrane


The proteins of the outer membrane of Gram-negative bacteria are critical for bacterialsurvival, from providing basic physiological functions, to aiding virulence and multidrugresistance. The Bam complex has been found to be essential for folding these proteins intothe outer membrane. The process by which this is achieved is unknown. Key to thisunderstanding is the knowledge of the structure of the complex and its individualcomponents. We aim to continue our investigation of the Bam complex by studying the complete BamABCDE complex and in particular identify how the individual components are organised on the membrane surface. To do this will use neutron reflectometry and specific deuteration to probe the structure of the Bam complex in a model E.coli outer membrane and elucidate the scaffolding of this essential complex on the membrane surface for the first time.

Metadata Access
Creator Dr Luke Clifton; Mrs Pooja Sridhar; Dr Mark Jeeves; Dr Tim Knowles
Publisher ISIS Neutron and Muon Source
Publication Year 2018
Rights CC-BY Attribution 4.0 International;
OpenAccess true
Contact isisdata(at)
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2015-04-22T08:00:00Z
Temporal Coverage End 2015-04-24T08:00:00Z