The data presented herein originates from a mesocosm study conducted as part of the EU H2020 OceanNETs project, aimed at investigating the ecological ramifications of ocean alkalinity enhancement. Nine mesocosms were deployed in Taliarte Harbour, Gran Canaria, Spain, and systematically sampled using integrated water samplers over the period spanning from September 10th to October 25th, 2021. Alkalinity was employed in a gradient design, ranging from ambient (0 µeq kg-1 added alkalinity, OAE0) to elevated levels of 2400 µeq kg-1 additional alkalinity (OAE2400) in increments of 300 µeq kg-1. The dataset encompasses a spectrum of sediment trap particle flux data, water column biogeochemistry variables, including inorganic nutrients, carbonate chemistry parameters, and particulate matter, alongside chlorophyll a concentrations. The study and data set offer insights into impacts of alkalinity enhancement on marine ecosystems and their associated biogeochemistry.