In this study, we used comparative transcriptomics to understand how oysters from either end of the species range maintain homeostasis across three acclimation temperatures 10, 20, and 30 C. With this approach, we identified genes that were differentially expressed in response to temperature between individuals of C. virginica collected from New Brunswick, Canada and Louisiana, USA. We observed a core set of genes whose expression responded to temperature in both populations, but also an even larger set of genes with expression patterns that were unique to each population.