In the urchin barren coast, recovered macroalgal stands highly relies up on the components of macroalgal seed bank. In the present study, we investigated the macroalgal seed banks using NGS based DNA meta-barcoding on shell surface of limpet Niveotectura pallida as the alternatives of natural cobbles. Twenty-three limpets were collected in four different zones (surf, turf algal, urchin barren and sandy zones) and two seasons (summer and winter) from urchin barren coasts in Sashigahama, Miyagi prefecture, Japan. In the rarefied data, totally 397 OTUs were obtained belonging to 8 families of Chlorophyta, 14 families of Phaeophyta and 40 families of Rhodophyta. In summer, the number of OTUs gradually increased from algal zone (41.4 ? 11.1 OTUs) to sandy zone (77.0 ? 15.2 OTUs) excepting for surf zone (50.0 ? 3.7 OTUs). On contrary, the number of OTUs are mostly similar in winter. The laminarialean OTUs were detected in all zone, while the sargassacean OTUs were found only in summer surf, turf and sandy zone. The Shannon-Wiener diversity index (H') was also the highest in summer sandy zone, but significant difference was not detected (p > 0.10). Given the data of this study and our previous study, higher number and variation of OTUs are obtained in the deeper zone. Thus, the macroalgal restoration should be carried out in the deeper zone.