Hydration of Guanosine, G-quartets and G-quadruplex

DOI

Guanosine (GMP) is the ideal brick for controlled self-assembly of biocompatible materials, as it has the ability to self-associate in water to form G-quartets (G4), and four stranded structures, termed G-quadruplexes (G4n). Recently we have studied on NIMROD (RB1810131) its hydration properties at concentrations of 0.117 M and 0.132 M and found that at the lowest concentrations GMP molecules are well hydrated and apart one from each other. Conversely, at the highest concentration we have found clear evidence for G4 aggregates, with structure compatible with NMR data. We request beam time on NIMROD in order to complete the study with an experiment on highly concentrated GMP solutions in 1 M KCl+water, where G4n aggregates are predominant, in order to extract the structure and hydration of G4n units and shed light on the role of hydrophobic/hydrophilic interactions in self-assembling.

Identifier
DOI https://doi.org/10.5286/ISIS.E.RB1910108-1
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/101144871
Provenance
Creator Dr Mariani; Dr Silvia Imberti; Professor Maria Antonietta Ricci; Professor Fabio Bruni; Dr Michael Di Gioacchino
Publisher ISIS Neutron and Muon Source
Publication Year 2022
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Natural Sciences; Physics
Temporal Coverage Begin 2019-03-09T09:00:00Z
Temporal Coverage End 2019-03-19T10:02:10Z