Determination of global decay rates of yeast transcriptome and identification of factors impact mRNA stability

In this work, we determine total mRNA decay rates in rpb1-1 and rpb1-1/caf1? cells, calculate half-lives in rpb1-1/caf1? cells relative to rpb1-1 cells and correlate them with codon optimality. Overall design: mRNA profiling was done on 10 time points in rpb1-1/caf1 cells and sequenced using a paired end protocol on an Illumina HiSeq2000 instrument. A biological duplicate was performed.

Identifier
Source https://data.blue-cloud.org/search-details?step=~012FFF6FE45375713162EF786816536CFC4990AACB3
Metadata Access https://data.blue-cloud.org/api/collections/FFF6FE45375713162EF786816536CFC4990AACB3
Provenance
Instrument Illumina HiSeq 2000; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Contributor Center for RNA Science and Therapeutics, Case Western Reserve University
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2018-06-26T00:00:00Z