De Novo Transcriptome Assembly and Gene Expression Profiling of the Copepod Calanus helgolandicus

Copepods are fundamental components of zooplankton and aquatic pelagic food webs, but reports on molecular responses linked to harmful phytoplankton species are still scarce. We present the de novo transcriptome of Calanus helgolandicus females feeding on the oxylipin-producing diatom Skeletonema marinoi. Differential expression analysis was also performed between copepod females exposed to the diatom and the control dinoflagellate Prorocentrum minimum, which does not produce oxylipins. Key transcripts involved in metabolism of carbohydrate, amino acids, folate and methionine, as well as in embryogenesis, signaling and response to stimulus, were differentially expressed in the two conditions. Expression levels of selected genes belonging to these functional categories were also analyzed by RT-qPCR in C. helgolandicus females exposed to the oxylipins polyunsaturated aldehydes (PUAs) heptadienal and octadienal. The results confirmed RNA-Seq and differential expression analysis. Our results offer new insights on the mechanism of action of oxylipins on maternally-induced embryo abnormality and may also help identify biomarker genes associated to diatom-related reproductive failure in natural copepod population at sea.

Identifier
Source https://data.blue-cloud.org/search-details?step=~012FD27650EFD7D7251A50414EE6432AC41DB0E6423
Metadata Access https://data.blue-cloud.org/api/collections/FD27650EFD7D7251A50414EE6432AC41DB0E6423
Provenance
Instrument Illumina HiSeq 2000; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Contributor Stazione Zoologica Anton Dohrn di Napoli
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2012-05-01T00:00:00Z