Nitrogen (N) is an essential nutrient element that affects the establish of stability of mangrove ecosystems. Despite the extremely importance of biological nitrogen fixation (BNF) mediated by diazotrophs in mangrove ecosystems, current studies generally overlook the influence of arbuscular mycorrhizal fungi (AMF) on diazotrophs-driven N fixation ecological processes. Here, we conducted a comprehensive survey on root-associated AMF and their interspecific interactions with diazotrophs in mangrove ecosystems.