The dataset consists of a list of treatments and relative variables that were varied in an artificial organo-mineral system according to earthworm activity and survival. Data was collected within the BAM! project (Bio-Accelerated Mineral Weathering), examining the potential of soil organisms to enhance mineral weathering for carbon sequestration. Data was obtained within 8 rounds of column-experiments carried out between March 2022 and September 2023. The dataset refers to the list of treatments and relative variables that were used to determine the main factors governing earthworm survival and activity in an artificial organo-mineral mixture, including percentages of mineral type and grain size, organic matter type, water flow rate and watering frequency, earthworm number and earthworm species, biochar and enzymes addition.