Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea


This image dataset provides ultrastructural micrographs of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected for study of microbial symbionts and histology during expedition JR17003a (RRS James Clark Ross, 2018) in Prince Gustav Channel, western Weddell Sea. Formalin-fixed sponge tissues were rinsed twice in PBS and postfixed in 1% osmium tetroxide in phosphate buffer for 6 hours at 4°C. Tissues were then rinsed twice in distilled water, dehydrated through to 100% ethanol and embedded in epoxy (TAAB 812). Ultrastructural sections of 60 nm were cut with a Leica Ultracut T microtome, stained in uranyl acetate and lead citrate, and then viewed and photographed with a Philips Morgagni transmission electron microscope equipped with a Gatan CCD camera. Image files are labeled with expedition, station and sample ID. Each image includes a scale.

DOI https://doi.org/10.1594/PANGAEA.966335
Related Identifier IsPartOf https://doi.org/10.1594/PANGAEA.965868
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.966335
Creator Leys, Sally P ORCID logo; Federwisch, Luisa (ORCID: 0000-0002-4815-475X)
Publisher PANGAEA
Publication Year 2024
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Resource Type Dataset
Format text/tab-separated-values
Size 360 data points
Discipline Earth System Research
Spatial Coverage (-58.478W, -64.058S, -57.501E, -63.616N); Prince Gustav Channel
Temporal Coverage Begin 2018-03-05T14:18:00Z
Temporal Coverage End 2018-03-07T17:54:00Z