KOSMOS 2020 Peru mesocosm study on ecosystem responses to different light and upwelling intensities: HPLC pigment concentrations and phytoplankton community composition based on CHEMTAX analysis of water samples


This data is part of the BMBF project CUSCO (Coastal Upwelling Systems in a Changing Ocean). Here we report pigment concentrations and the phytoplankton community composition based on CHEMTAX analysis during a 35-day experiment, where we enclosed natural plankton communities in in-situ mesocosms off Peru. The experiment investigated the interactive effects of light and upwelling on the Humboldt upwelling ecosystem by mimicking a gradient of upwelling intensities (0%, 15%, 30%, 45% and 60%) under summer-time high light and winter-time low light. Integrated seawater samples from a depth between 0 and 10m were collected using a 5L Integrating Water sampler (IWS; Hydro-Bios, Kiel). To obtain pigment concentrations 0.15 – 1L of water sampled from the water column were filtered onto glass-fiber filters (precombusted at 450°C for 6h, GFF, 0.7 µm nominal pore size, Whatman) using a low vacuum of 200 mbar. Afterwards the filters were stored in cryovials at -80°C until analysis of photosynthetic pigments using reverse-phase high-performance liquid chromatography (HPLC) following (Barlow et al., 1997; doi:10.3354/meps161303) as described by (Paul et al., 2015; doi:10.5194/bg-19-5911-2022). The phytoplankton community composition was calculated with CHEMTAX, which classifies phytoplankton taxa based upon taxon-specific pigment ratios (Mackey et al., 1996; doi:10.3354/meps144265). The values for the Peruvian upwelling system determined by (DiTullio et al., 2005; doi:10.4319/LO.2005.50.6.1887) as described by (Meyer et al., 2017; doi:10.3389/fmars.2017.00001) were used as pigment ratios. As phytoplankton microscopy revealed high abundances of the raphydophite Fibrocapsa japonica, the initial pigment matrix was complemented by ratios for F. japonica from (Laza-Martinez et al., 2007; doi:10.1093/plankt/fbm069) from the Atlantic.

Mesocosm 10 describes the control sample from the PacificHPLC data for day3 - mesocosm3 was measured as triplicate and day 7 - mesocosm 9 as duplicate. The values provided are the averages of the measured replicates.

DOI https://doi.org/10.1594/PANGAEA.961210
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.961210
Creator Behncke, Jaqueline; Thielecke, Antonia ORCID logo; Kittu, Leila ORCID logo; Georgieva, Silvia; Nachtigall, Kerstin; Hansen, Levka; Fernández-Méndez, Mar ORCID logo
Publisher PANGAEA
Publication Year 2023
Funding Reference Federal Ministry of Education and Research https://doi.org/10.13039/501100002347 Crossref Funder ID 03F0813A https://foerderportal.bund.de/foekat/jsp/SucheAction.do?actionMode=view&fkz=03F0813A Küstennahe Auftriebsgebiete CUSCO: Auswirkungen geänderter Auftriebsintensitäten auf Struktur, Funktion und Produktivität pelagischer Nahrungsnetze im peruanischen Humboldt-Auftriebsgebiet; Horizon 2020 https://doi.org/10.13039/501100007601 Crossref Funder ID 731065 https://cordis.europa.eu/project/id/731065 Network of Leading European AQUAtic MesoCOSM Facilities Connecting Mountains to Oceans from the Arctic to the Mediterranean
Rights Data access is restricted (moratorium, sensitive data, license constraints)
OpenAccess false
Resource Type Dataset
Format text/tab-separated-values
Size 5484 data points
Discipline Earth System Research
Spatial Coverage (-77.236W, -12.056S, -77.235E, -12.055N)
Temporal Coverage Begin 2020-02-28T00:00:00Z
Temporal Coverage End 2020-04-02T00:00:00Z