TEM and Confocal Microscopy Images of Polystyrene Nanoplastics (PS-NPLs) with Different Sizes and Surface Functionalizations in HUVECs

Nanoplastic Types:

Carboxylated PS (PS-C): 30 nm, 50 nm, and 100 nm Aminated PS (PS-A): 50 nm Pristine PS (PS-P): 50 nm

For confocal microscopy, fluorescently labeled PS-NPLs were used. For TEM, non-labeled PS-NPLs were used, except for the 30 nm PS-C, where only the labeled version was available.

List of PS-NPLs used from PlasticHeal project: Fluorescently labeled (YG): PS-C 30 nm YG: PH_PS-C-30-FLY PS-C 50 nm YG: PH_PS-C-50-FLY PS-C 100 nm YG: PH_PS-C-100-FLYb PS-P 50 nm YG: PH_PS-P-50-FLY PS-A 50 nm YG: PH_PS-A-50-FLY

Non-labeled: PS-C 50 nm: PH_PS-C-50 PS-C 100 nm: PH_PS-C-100b PS-P 50 nm: PH_PS-P-50a PS-A 50 nm: PH_PS-A-50

Methodology from research article: Martín-Pérez, J., Villacorta, A., Banaei, G., Morataya-Reyes, M., Tavakolpournegari, A., Marcos, R., Hernández, A., & García-Rodriguez, A. 2024. Hazard assessment of nanoplastics is driven by their surface-functionalization. Effects in human-derived primary endothelial cells. The Science of the total environment, 934, 173236. https://doi.org/10.1016/j.scitotenv.2024.173236