In this study, we determined the target spectrum of the VqmR sRNA via vqmR pulse-expression followed by RNA-sequencing. Overall design: mRNA profiles of V. cholerae dvqmR strains carrying pBAD-ctr (empty vector control) or pBAD-vqmR (inducible sRNA expression vector). The bacteria were grown to exponential phase, expression of vqmR was induced by addition of L-arabinose, and RNA-samples were taken 15 minutes after induction. Three independent biological replicates for each condition are provided.