Five iron mat communities were sampled with a hydraulic biosyringe suction pump in the Jan Mayen hydrothermal vent fields (71°N 6°W). The sampling method led to two phases a solid bottom fraction (raw, R) and a liquid (top, T) part, that were both sampled separately. Two different hydrothermal conditions were considered high-flux hydrothermal venting (warm, W) as opposed to diffuse fluid flows (cold, C).