This BAG is composed of 6 working groups from 3 locations (university of Cologne, Bochum, Düsseldorf), the projects for this proposal are rather heterogeneous. Nonetheless, we are focusing on 6 main topics: 1.) proteins involved in transport processes, transporters or channels, either embedded in the membrane or acting in the cytosol (e.g. ABC transporters, fusion proteins, regulators) 2.) proteins involved in the immune system, pathogen invasion or defense/resistance processes (dehydratases, toxins, binding proteins) 3.) proteins involved or associated with diseases like cancer or developmental disorders (e.g. kinase, toxins) 4.) enzymes of putative industrial use to encounter increasing environmental pollution (e.g. transaminases, estereases) 5.) proteins involved in light harvesting, photosynthesis (C3/C4 pathways) and chromophore binding (e.g. synthases, photosensor domains) 6.) signaling or transport processes (e.g. ethylene, collagen) or fixation ([Fe-Fe]-hydrogenases)