Assembling Biomimetic layers on monolayers of Gold Nanoparticles

DOI

Gold provides a high quality surface on which to arrange biomolecules such as proteins since we can use cysteine amino acids to deliver gold thiol chemical bonds which can orient the proteins in a defined manner. Whilst a thin layer of gold can be produced by sputtering this is opaque and limits the use of these surfaces in microscopy which is a major component of biological analysis. In collaboration with Orla Protein Technologies Ltd we are studying glass slides upon which a layer of gold nanoparticles has been assembled. Biomolecules can be assembled on these layers which are being measured by a range of technique including electron microscopy, XPS and AFM but only neutron reflectivity can image and provide a profile of the density and distribution of all the components. We hope to use neutron reflectivity in conjunction with QCM-D which can measure the mass increases directly.

Identifier
DOI https://doi.org/10.5286/ISIS.E.73943597
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/73943597
Provenance
Creator Dr Maxmilian Skoda; Professor Jeremy Lakey; Mr Timothy Robson; Dr Nico Paracini; Dr Luke Clifton
Publisher ISIS Neutron and Muon Source
Publication Year 2019
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2016-02-29T09:00:00Z
Temporal Coverage End 2016-03-02T09:00:00Z