-
mini PCR: effects of PCR rxn volume and gDNA input validation
part of mackerel paper -
SM Apprill WHOI SeaWater
We investigated dynamics between corals and seawater bacterioplankton during the summer of 2013 using aquaria-based experiments at the Bermuda Institute of Ocean Sciences... -
2018 Australia fish microbiome study
Atl salmon study ABSTRACT Successful rearing of fish in hatcheries is critical for conservation, recreational fishing, and commercial fishing through wild stock enhancements,... -
Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribe...
combined samples sequenced with primers modified for forward barcoding on the 515f site, extended to include V5 and improved ITS -
DNA extraction comparison testing 2019-2020
Our DNA extraction protocol is years old, and we want to compare new extraction methods to address outstanding issues. For more details, check out the motivation and... -
Multi-omics of host-gut microbiota interactions in healthy and parasitized fa...
Animals and their associated microbiota share long evolutionary histories. However, it is not always clear how host genotype and microbiota interact to affect phenotype. We... -
3D multi-omic profiling of an assemblage of Hawaiian corals
Samples were taken from species Porities lobata, Montipora capitata, Montipora flabellata and subjected to 16S sequencing for bacterial profiling and LC-MS/MS for metabolomic... -
Knight skin biogeography comparison
Knight_skin_biogeography - Comparison of skin samples from a rat (mammal), cyclostomid (fish), iguana (reptile), bull frog (amphibian) and a bird (pigeon) to determine if the... -
Earth Microbiome Project Multi-omics (EMP500)
The Earth Microbiome Project Multi-omics component (EMP500) involves amplicon and shotgun metagenomic sequencing and metabolomic profiling of over five hundred microbial... -
Genetic diversity of viral arsM in arsenic-contaminated soil during 15-day fl...
These soils contain up to 2800 mg/kg of TOTAL arsenic These data are based on amplicon sequencing (arsM-F1 and arsM-R2). -
Microbial effects of livestock manure fertilization on freshwater aquaculture...
The majority of seafood is produced from farming, with most finfish coming from freshwater ponds. Across continents, aquaculture is growing fastest in Africa at 11.7 % annual... -
Song Colorado freshwater fish
Microbiota of slime and gut samples from different species of freshwater fish from the Colorado River. -
Fish microbiota 101: host traits, ecology, and environmental factors influenc...
Fish are the most diverse and widely distributed vertebrates, yet little is known about the microbial ecology of fishes nor the biological and environmental factors that... -
Knight_extraction_test
One plate of samples was extracted using several different extraction tools (new, faster) for comparison with the existing pipeline -
Microbial diversity in arctic freshwaters is structured by inoculation of mic...
A 12-year sample archive of DNA from Alaskan arctic tundra ecosystems are available to the Earth Microbiome Project for comparison of microbial diversity across a range of... -
Tijuana River pollution at Imperial Beach 2019
Rain events increase Tijuana river flow to levels that the international water treatment plant in the Mexico-US border can not process. Therefore, rain runoff and sewage... -
Patterns of microbiome transition along salinity gradients, and importance fo...
We are interested in whether the host fish microbiome is important for host adaptation to alternate osmotic niches. Toward this goal, we seek to describe the patterns of... -
Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary
Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation. However,... -
The Sponge Microbiome Project
Marine sponges (phylum Porifera) are a diverse, phylogenetically deep-branching clade known for forming intimate partnerships with complex communities of microorganisms. To...