35 datasets found

Here we present an analysis of latitudinal genomic variation in Drosophila melanogaster occurring in parallel in North America and Australia. We find evidence of substantial,...

Pooled resequencing of Drosophila melanogaster collected across Europe and across two seasons of one year.

We performed ChIP-Seq on MV4 11 cells after 6 hours treatment of DMSO, 100nM and 200nM THZ531 to determine the location of Pol II with phosphorylated Ser2 occupancy Overall...

In organisms with complex life cycles, life stages that are most susceptible to environmental stress may determine species persistence in the face of climate change. Early...

A comparison of somatic and germline small RNA pathways across the arthropods

Background: The interplay between hosts and their associated microbiome is now recognized as a fundamental basis of the ecology, evolution and development of both players. These...

Whole-genome DNA-seq and strand-specific RNA-seq on 18 species, including 14 metazoans and four unicellular eukaryotes closely related to metazoans. For each species, two to...

Study somatic mutation in animal tissues, including mutation burden accumulation through lifespan and mutational signatures.

Salmonella enterica genomes sequenced as part of PulseNet USA surveillance for foodborne disease

We investigate tissue transcriptomic evolution across bilaterian animals by analyzing RNA-seq data from eight different tissues across twenty species. Overall design:...

Eukaryotic cytosine methylation represses transposable elements, but also occurs in bodies of active genes. The extent to which these processes are conserved is unclear, and...

Investigating the evolution and function of DNA methylation across arthropods

We report the comprehensive sequencing of small RNA libraries created from different developmental stages of the basal chordate, Ciona intestinalis. Surprisingly, half of the...

Heteromorphic sex chromosomes induce potentially deleterious gene expression imbalances that are frequently corrected by dosage compensation (DC). Three distinct molecular...

The discovery of N6-methyldeoxyadenine (6mA) across eukaryotes led to a search for additional epigenetic mechanisms. However, some studies have highlighted confounding factors...

Banana samples fed on by wild Drosophila spp. were collected, and the fungal or bacterial compositions of their microbiota was analyzed by ITS or 16S amplicon sequencing,...

Here we present dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq), which encodes DMS modifications as mismatches using a thermostable group II intron reverse...

The genomes of several insect species were sequenced and their X-chromosomes characterized.

Metazoan promoters are highly enriched in secondary structure forming motifs, among which G-quadruplexes (G4s). We describe G4access, a novel approach to isolate and sequence...

Poly(A) tails enhance the stability and translation of most eukaryotic messenger RNAs, but difficulties in globally measuring poly(A)-tail lengths have impeded greater...