Genome-wide map of Pol II occupancy alteration on chromatin upon CDK12 and CDK13 inhibition (ChIP-Seq)

We performed ChIP-Seq on MV4 11 cells after 6 hours treatment of DMSO, 100nM and 200nM THZ531 to determine the location of Pol II with phosphorylated Ser2 occupancy Overall design: MV4 11 cells, DMSO, 100nM, 200nM THZ531 treatment for 6 hours, then Pol II bound chromatin were isolated from soniacated nuclear extracts using antibody angaist RNA polymerase II (phospho CTD Ser-2)

Identifier
Source https://data.blue-cloud.org/search-details?step=~012F3006C4E109BA44649C701DE7F5F8FC8A5C6B175
Metadata Access https://data.blue-cloud.org/api/collections/F3006C4E109BA44649C701DE7F5F8FC8A5C6B175
Provenance
Instrument Illumina MiSeq; NextSeq 500; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Contributor Peter MacCallum Cancer Centre
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Spatial Coverage (-164.041W, 74.366S, -158.524E, 77.071N)
Temporal Coverage Begin 2016-07-13T00:00:00Z
Temporal Coverage End 2020-05-01T00:00:00Z