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Rtt109 effect on replication speed via histone acetylation
The wrapping of DNA around histone octamers challenges processes that use DNA as their template. In-vitro, DNA replication through chromatin depends on histone modifiers,... -
Chip histone H3pT11 in glc7-ts in yeast cells
Genome-wide detection of histone H3pT11 binding in BY4741 and glc7-ts cells,and binding to telomere genes and autophagy genes -
3D nuclear organization in Saccharomyces cerevisiae with megachromosomes
Eukaryotic genomes vary greatly in terms of size, chromosome number and genetic complexity. Their temporal organization in the nucleus is complex, reflecting coordination... -
Modulating DNA repair pathways to diversify genomic alterations in Saccharomy...
Nuclease based genome editing systems have been engineered as powerful tools to drive genomic alterations and enhance genome evolution via precise engineering in human and... -
Genome-scale engineering of Saccharomyces cerevisiae with single-nulceotide p...
Through systematically exploring the fitness landscape in a genome-wide manner, genome-scale engineering can test multiple hypotheses in parallel and overcome our limited... -
Expression level is a major modifier of the fitness landscape of a protein co...
The phenotypic consequence of a genetic mutation depends on many factors including the expression level of a gene. However, a comprehensive quantification of this expression... -
Saccharomyces cerevisiae BY4741 Raw sequence reads
Adaptive isolates from 10 populations evolved for 150 and 300 generations in fluctuating Pi levels -
Overexpression profiling reveals cellular requirements in context of genetic ...
Systematic identification of genes whose overexpression functions adaptively in S. cerevisiae to reveals the mechanisms of adaptation driven by overexpression. We uploaded the... -
Full-length RNA sequencing using Illumina and Nanopore technologies
Here we show a method that sequences full-length cDNA by combining SMARTer with the ONT MinION sequencer. We sequenced the RNA in two different biological samples of... -
Saccharomyces cerevisiae Translation Massively Parallel Reporter Assay raw reads
A library of transcript leaders from S. cerevisiae and S. paradoxus was cloned upstream of a YFP reporter gene to quantify their effects on translation. Mutant versions were... -
Adaptive Prediction Emerges Over Short Evolutionary Time Scales
The ability of microorganisms to conditionally adapt to future conditions, a behavior termed adaptive prediction (AP), erodes rapidly when the structure of their environment... -
Systematic Identification of Genes whose Overexpression is Functionally Adaptive
A project to systematically identify budding yeast genes whose overexpression is adaptive in a variety of environmental and genetic contexts. Sequencing data obtained using ONT... -
A genome-wide screen reveals a role for the HIR histone chaperone complex in ...
Centromeric localization of Cse4 (CENP-A in humans) is essential for faithful chromosome segregation. Overexpression and mislocalization of Cse4 and its homologs leads to... -
Transcriptomics analysis of octanoic acid producing strain in comparison to w...
Transcriptomics analysis of an octanoic acid producing strain at three different time points (early exponential phase, early stationary and late stationary phase) in comparison... -
Extrachromosomal circular DNA is common in yeast.
Examples of extrachromosomal circular DNAs (eccDNAs) are found in many organisms but their impact on genetic variation at the genome scale has not been investigated. We mapped... -
Saccharomyces cerevisiae Growing on Xylulose Expression Data
This dataset consists of gene expression data for BY4741 wild type and deletion mutants during logarithmic growth on glucose, galactose and xylulose. -
Saccharomyces cerevisiae BY4741 Raw sequence reads
The data is used for detecting the genome variation after genome engineering. -
Genome-wide base editor screen identifies regulators of protein abundance in ...
Results from CRISPR base editor screens in yeast to study the effects of mutations on canavanine resistance, on cell fitness, and on the abundance of 11 individual proteins. See... -
Saccharomyces cerevisiae BY4741 derivatives with tRNA neochromosome raw reads
The tRNA neochromosome contains all 275 nuclear tRNAs. The data consolidated here is linked to the publication describing the design, construction and functional...