Further SANS contrast measurements on important protein/DNA interactions from RCaH

DOI

This proposal is to perform further SANS contrast measurements on three protein/DNA interactions currently worked on at the Research Complex at Harwell. This is part of an ongoing productive relationship between the two facilities, coordinated by Dr. David Scott of the University of Nottingham funded by ISIS to sited in the RCaH 2 days a week.The systems understudy all form part of crystallographic studies as part of an MRC programme grant to Prof. Simon Phillips, the RCaH Director, and all are important in the fundamental processes of DNA repair, recombination and replication. Preliminary data is available from SAXS and analytical ultracentrifugation measurements showing that the systems are stable in D2O and monodisperse. Preliminary SANS data through SANSexpresses has shown that suitable concentration ranges are attainable for further SANS measurements.

Identifier
DOI https://doi.org/10.5286/ISIS.E.58446084
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/58446084
Provenance
Creator Miss Nishtha Gulati; Dr Stephen Carr; Mr Matthew Lawes; Dr Gaye White; Dr DENIS PTCHELKINE; Mr Matthew Green; Dr Tom Clarke; Dr David Scott; Dr Marcus Edwards; Dr Sarah Rogers; Dr Richard Heenan
Publisher ISIS Neutron and Muon Source
Publication Year 2018
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2015-04-16T08:00:00Z
Temporal Coverage End 2015-04-17T08:00:00Z