The red cusk-eel (Genypterus chilensis) is an abundant fish species distributed along the coasts of the Southeastern Pacific Ocean, which has been considered one of the endemic species of greatest acuaculture diversification potential in Chile. The objective of the study was to determine the effect of thermal stress in the liver of red cusk-eel, determine the transcriptional regulation of pathways in response to this stressor as well as identify thermal stress biomarkers in this species, relevant information under a climate change scenario. For this we use RNA-seq of liver of control and thermal stress groups with Illumina Hiseq technology.