In order to explore the bacteriostatic mechanism of Neolamarckia cadamba essential oil, the effect of Neolamarckia cadamba essential oil on microbial transcription in logarithmic growth phase was determined. Overall design: The transcriptional group of three kinds of microorganisms(Bacillus circulans,Candida glabrata,Aspergillus flavus) treated with Neolamarckia cadamba essential oil for four hours was determined, and the control group was set up.