Renal denervation (RDN) can improve cardiac function by ameliorating aberrations of gut microbiota, and non-alcoholic fatty liver disease (NAFLD) is associated with gut microbiota dysbiosis and critically involved in the development of heart failure (HF). It is unclear whether the beneficial role of RDN on gut microbiota in HF can be affected by NAFLD. In our study, HF Sprague-Dawley rats were fed high-fat diet and underwent RDN. Sequencing of 16S rRNA gene in fecal samples was detected.