Ecohydrological information was collected during the period 2013 to 2015 as part of the project "Ecohydrological observations to improve hydrological models estimates in the Sonora River Basin." This project was managed by the Sonora Institute of Technology (ITSON), the University of Sonora (UNISON), Arizona State University (ASU), the New Mexico Institute of Mining and Technology (NMT) and was funded by the National Science Foundation (NSF), the National Oceanic and Atmospheric Administration (NOAA) and the National Council of Science and Technology (CONACYT). Data were obtained at the Sierra Los Locos ecohydrological monitoring station, located at the watershed divide of the Sonora and San Miguel river basins in the state of Sonora, Mexico. This station was established with the objective of acquiring data of ecohydrological importance that helps the parameterization, validation and calibration of hydrological and vegetation models. Sap flow measurements focused on the two dominant species of oaks in the region: Blue Oak (Quercus oblongifolia) and Chihuahuan Oak (Quercus chihuahuensis). To obtain sap flow data, two pair of sensors were installed in each tree to estimate the voltage or temperature differential based on Granier's principle (Granier, 1985). The differential voltage (mV) data were processed using the Baseliner Version 4.0 package (Oishi et al., 2016) to estimate sap flow values. In addition, measurements of meteorological variables and soil humidity/temperature conditions at different depths within the site were included. The data set contains different variables measured in a 30-minute interval during the period 2013-2015 using several sensors installed in the study site (see related datasets).