As a new group of synthetic biosurfactants, the acyl-L-carnitines offer potential for different applications including working as edible biocides. Whilst extensive studies have examined their biological functions, there is little work to look at their physical properties which crucially underpin their membrane lytic actions against different microorganisms. In the past decade, there is a rise in diseases caused by bacterial infections. As a result, there is a high demand for antibacterial agents which can kill bacteria effectively whilst remaining biocompatible to the hosts. The aim of this project is to examine how this group of new surfactants aggregate in aqueous solutions under different pH and ionic strength.