This dataset contains the experimental data associated to the publication "A novel QTL region for pH and meat colour in Duroc pigs" by Molinero et al. (2024; https://doi.org/10.1111/age.13426). It includes a TXT file with the genotypes obtained using real-time PCR with High-Resolution Melt and the phenotypic data necessary for the validation of the effect of single nucleotide polymorphisms rs330928088 (at gene PHKG1), rs326497155 (at chromosome SSC8), and rs1108399077 (at gene PRKAG3) on pH and meat colour in 828 pigs.