The study aims to conduct whole-genome resequencing of Trichoderma harzianum, a widely used biocontrol agent in agriculture, with the goal of elucidating its genetic diversity, population structure, and potential for strain improvement. By analyzing the genomic variations among different strains of T. harzianum, researchers seek to identify key genes associated with beneficial traits such as antagonistic activity against plant pathogens, production of bioactive compounds, and tolerance to various environmental stresses. Understanding the genomic landscape of T. harzianum holds significant relevance for agriculture and biotechnology, as it can facilitate the development of enhanced strains with improved biocontrol efficacy, ultimately contributing to sustainable crop production and reduced reliance on chemical pesticides.