Acute cholecystitis (AC) is the acute inflammation of the gallbladder. AC is usually associated with cholelithiasis, with gallstones detected in >90% cases. Additional causes include circulation defect of the gallbladder wall, bacterial infection, and chemical disorder. The guidelines recommend treatment based on the severity, with patients generally treated with antimicrobial agents before operation, drainage, or general care is considered. When conservative treatment with drainage is selected, antimicrobial agents are administered depending on the bile culture examination and antimicrobial susceptibility testing results. In this study, we isolated bacteria strains from bile samples of AC patients, and analyzed whole genome sequencing of these strains.