Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments


On four occasions during the two year incubation of a subantarctic E. huxleyi culture (2015-11-20 to 2017-12-01), from Day 309 onwards, cell size of the cultures was measured at cross-over points, after 7 – 8 generations while still in exponential growth phase. Cells from Now (11°C and pH 8.1) and Future (14°C and pH 7.8) cultures, and also the crossover experiments - Future cells inoculated into Now medium and incubated under Now conditions (Future in Now), and Now cells incubated in Future medium under Future conditions (Now in Future) were fixed in 1 % glutaraldehyde. Subsamples were mounted in a nanoplankton chamber (Phytotech) and measured with a calibrated eye-piece graticule under an Olympus IX70 inverted microscope. As the fixation process caused coccoliths to separate, the cell size measurements were for the cell body only, which allows more accurate determinations of cell volume (Buitenhuis et al., 2008). Cell volume was calculated assuming that the cells were spherical.

The study was supported by Coasts and Oceans Centre (Strategic Science Investment Fund of the National Institute of Water and Atmospheric Research).

DOI https://doi.org/10.1594/PANGAEA.959827
Related Identifier https://doi.org/10.1594/PANGAEA.959857
Related Identifier https://doi.org/10.1371/journal.pone.0284415
Related Identifier https://doi.org/10.4319/lo.2008.53.3.1181
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.959827
Creator Armstrong, Evelyn (ORCID: 0000-0001-8143-027X); Law, Cliff S ORCID logo
Publisher PANGAEA
Publication Year 2023
Funding Reference University of Otago https://doi.org/10.13039/100008247 Crossref Funder ID ORF_0115-0316_Law University of Otago Research Grant; University of Otago https://doi.org/10.13039/100008247 Crossref Funder ID ORF_0117-0318_Law University of Otago Research Grant
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Resource Type Dataset
Format text/tab-separated-values
Size 285 data points
Discipline Earth System Research
Spatial Coverage (171.532 LON, -45.829 LAT)