Viral, bacterial and ciliate abundance and viral-bacterial diversity in mesocosm experiments, 2007-2008, Kongsfjorden


Two mesocosm experiments, PAME-I and PAME-II were conducted in 2007 and 2008 to investigate fate of organic carbon in the arctic microbial food web. Mesocosms were nutrient fertilized initially to induce phytoplankton bloom development. In PAME-I eight units (each 700 L) formed two four point gradients of additional DOC in form of glucose (0, 0.5, 1 and 3 times Redfield ratio in terms of carbon relative to the nitrogen and phosphorus additions) (Fig. 1). All the eight units also got a daily dose of NH4+ and PO43- in Redfield ratio. Two gradients were set up, one with silicate addition, performed in the Arctic location Ny Ålesund, Svalbard, have previously been reported to give different food-web level responses to similar nutrient perturbations. In PAME-II all ten units (each 900 L) formed two four point gradients of additional DOC in form of glucose (0, 0.5, 1, 2 and 3 times Redfield ratio in terms of carbon relative to nitrogen and phosphorus additions). The two gradients in glucose were kept silicate replete. NH4+ was used as the DIN source in one gradient (units 1 to 5) and NO3- in the other (units 6-9). All units got a daily dose of PO43- in Redfield ratio. Prokaryotes and viruses were measured by flow cytometry, while ciliate abundances were counted using a Flow Cam. Viral and bacterial diversity was measured by PFGE and DGGE, respectively. In PAME-II the abundance of ciliates was lower than in PAME-I, presumably caused by higher copepod grazing. The abundances of prokaryotes and viruses were also lower in PAME-II compared to PAME-I. Further, less diversity was detected in the viral community (FCM and PFGE) in PAME-II, and no response was observed in the bacterial community structure due to addition of organic carbon.

This dataset is part of PAME:

Supplement to: Sandaa, Ruth-Anne; Pree, Bernadette; Larsen, Aud; Våge, Selina; Töpper, Birte; Töpper, Joachim P; Thyrhaug, Runar; Thingstad, Tron Frede (2017): The Response of heterotrophic prokaryote and viral communities to labile organic carbon inputs is controlled by the predator food chain structure. Viruses, 9(9), 238

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Metadata Access
Creator Sandaa, Ruth-Anne; Larsen, Aud ORCID logo; Thyrhaug, Runar; Egge, Jorun K; Töpper, Birte
Publisher PANGAEA
Publication Year 2016
Rights Creative Commons Attribution 3.0 Unported;
OpenAccess true
Resource Type Supplementary Dataset; Dataset
Format text/tab-separated-values
Size 50 data points
Discipline Earth System Research
Spatial Coverage (11.956 LON, 78.954 LAT); Svalbard
Temporal Coverage Begin 2007-08-02T00:00:00Z
Temporal Coverage End 2008-07-10T00:00:00Z