Dynamics of the myoglobin-sucrose-water system for understanding the role of cryoprotectants

DOI

We intend to study the dynamics of the myoglobin-sucrose-water system, with the aim to understand why trehalose exhibits better cryopreservation and stabilization properties than sucrose. We have already performed QENS studies on the corresponding trehalose system (RB 1600024), as well as NIMROD experiments on both systems (RB 1520094 and RB 1700070), with complementary EPSR modelling and MD simulations to guide us in the interpretations of the NIMROD and IRIS data, respectively. Here, we particularly aim to elucidate whether the differences between trehalose and sucrose can be explained by a smaller reduction of the protein dynamics in the case of sucrose. This experiment will furthermore be used as a “student study” in a neutron spectroscopy course within the Swedish national graduate school in neutron scattering (SwedNESS) and, therefore, would need to be scheduled on 26 Nov 2018.

Identifier
DOI https://doi.org/10.5286/ISIS.E.99689815
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/99689815
Provenance
Creator Miss Filippa Lundin; Ms Stefanie Siebeneichler; Miss Karolina Mothander; Mr Gustav Ek; Miss Judith Ingrid Marie Lycksell; Mr Paulo Henrique Barros Brant Carvalho; Professor Jan Swenson; Mr Christoffer Olsson; Dr Franz Demmel
Publisher ISIS Neutron and Muon Source
Publication Year 2021
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2018-11-26T09:30:00Z
Temporal Coverage End 2018-11-30T08:53:30Z