Bacteria and cyanobacteria abundance measured on water bottle samples during BAFFIN cruise BAF89/3

DOI

Bacterial countsSamples for enumeration of bacterial abundance were drawn from Niskin bottles and immediately preserved in 20 mL glass scintillation vials with 0.2 µm filtered (Millex-GS, Millipore Corp.) formalin (2% final concentration). Preserved samples were stored dark in a refrigerator. Each sample was poured over triplicate polycarbonate membranes (0.2 µm black Nuclepore) underlain by pre-wetted backing filters (Sartorius #11306) which aid in distributing cells in an even manner on the Nuclepore surface. Cells were stained for 10 minutes with DAPI at a final concentration of 2 µg/ml and then drawn through the filters at a vacuum pressure of about 150 mm Hg. The filters were examined by epifluorescence microscopy (Leitz Orthoplan) under ultraviolet excitation from a mercury arc lamp (HBO 100W).Cyanobacterial countsSamples for enumeration of cyanobacterial abundance (20-100 ml) were freshly filtered in triplicate onto 0.2 µm black Nuclepore membranes which were underlain by backing filters. The membranes were stored refrigerated after having been covered in a drop of immersion oil. Cyanobacteria were enumerated by epifluorescence microscopy under green excitation (Leitz block N2).

Identifier
DOI https://doi.org/10.1594/PANGAEA.808255
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.808255
Provenance
Creator Li, William K W
Publisher PANGAEA
Contributor Li, Bill
Publication Year 2013
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Representation
Resource Type Dataset
Format text/tab-separated-values
Size 306 data points
Discipline Earth System Research
Spatial Coverage (-61.280W, 31.980S, -39.990E, 47.000N); North Atlantic Ocean
Temporal Coverage Begin 1989-04-20T00:00:00Z
Temporal Coverage End 1989-05-10T00:00:00Z