Lipid exchange in circularized nanodiscs

DOI

Nanodiscs (NDs) are discoidal lipid-protein aggregates composed by a lipid bilayer surrounded by a protein belt. NDs are suitable systems to investigate membrane protein structure in solution by scattering (e.g. SANS and SAXS), spectroscopy (e.g. NMR) and Cryo-EM techniques. Recently, we developed three types of protein belts (named csE3, cs1D1 and csDH5), aiming at improving ND thermal and time stability for membrane protein structure characterization in solution. Here we propose to complement the structural characterization of csE3, cs1D1 and csDH5 NDs by investigating lipid-exchange between NDs emerged as a key dynamic aspect to determine the ND capability to mimic lipid membranes.

Identifier
DOI https://doi.org/10.5286/ISIS.E.RB1910265-1
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/101138412
Provenance
Creator Miss Abigail Barclay; Dr Alessandra Luchini; Mr Nicolai Johansen; Mr Frederik Tidemand; Dr Najet Mahmoudi; Professor Lise Arleth
Publisher ISIS Neutron and Muon Source
Publication Year 2022
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2019-03-25T09:00:00Z
Temporal Coverage End 2019-05-30T15:44:52Z