We used a controlled laboratory experiment to test the concurrent effects of projected future summer and winter ocean temperatures, ocean acidification, and novel species interaction on the behaviour and growth of range-extending tropical and co-shoaling native temperate fish species across a 38-day exposure period. Proxies for growth were somatic growth and length increase, while video recordings were used to examine a repertoire of behavioural proxies (aggression, activity, boldness and feeding).

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-9-11.