blocking PCR amplification of metazoan DNA for protistan diversity

Protists form the basis of marine food webs. However, the understanding of protistan diversity is typically impeded by the predominance of metazoans, especially when employing universal primers in samples from large size fractions. In order to block the amplification of metazoan DNA in PCR, we designed 3 blocking primers and tested the performance using pure cultures and environmental samples. Furthermore, blockimg primers were applied to samples from a large size fraction, which is insufficiently studied due to the dominance of metazoans.

Identifier
Source https://data.blue-cloud.org/search-details?step=~012FAE318E9FEE6D148CFF53656CA9962A69939B24D
Metadata Access https://data.blue-cloud.org/api/collections/FAE318E9FEE6D148CFF53656CA9962A69939B24D
Provenance
Instrument 454 GS Junior; LS454
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Spatial Coverage (123.000W, 23.500S, 128.000E, 23.500N)
Temporal Point 2013-07-01T00:00:00Z