To further define hypoxia response and confirm SrbA regulation of genes with associated SrbA DNA binding events in their promoter regions, RNA-seq analysis of the transcriptome was conducted pooling total RNA samples from independent biological cultures in triplicate. A 30 and 120 minute temporal response to hypoxia was examined by comparing wild type to normoxia, and deltasrbA and deltasrbB hypoxia to wildtype hypoxia.