Rhamnolipids are a type of glycolipid biosurfactant, produced as secondary metabolites by various Pseudomonas and Burkholderia species; they have been shown to possess anti-bacterial and anti-fungal activity. They can now be produced with high efficiency allowing for more economical production and industrial scalability. Numerous studies have reviewed the potential applications of biosurfactants that are useful for therapeutic applications that include cancer therapeutics and drug delivery systems. Their low toxicity, surface activity, and anti-mycotic properties (8) make them promising candidates for development of active delivery systems for anti-fungal drugs like amphotericin B (AmB). In the studies proposed here, our aim is to carry out small angle neutron scattering (SANS) experiments to determine the structure and stability of vesicles prepared from R1(/sterol) with and without AmB