The oyster production worldwide is continuously threatened by Ostreid herpesvirus 1 (OsHV-1), being its most virulent variants the main cause of high mortality rates with significant economic losses. However, little is known about host-pathogen interactions and genetic determinants of the OsHV-1 virulence.</p><p>We used an NGS process to sequence the whole genome of a sample recovered during laboratory-based in vivo experimental infection. The experimental infections started using an Italian OsHV-1 strain purified from naturally infected oysters farmed in Porto Tolle, North Adriatic Sea.