Wavelength-specific molar decadic absorption coefficients of various phosphatidylcholine lipid standards for wavelengths 200-500 nm. Lipids were dissolved in methanol and wavelength-specific absorbances were measured in 100 mm quartz cuvettes using a dual-path UV-visible spectrophotometer (Thermo Nicolet Evolution 300; ThermoFisher Scientific).
Phosphatidylcholine 22:0, 22:0 (1,2-dibehenoyl-sn-glycero-3-phosphocholine) = PC 22:0, 22:0Phosphatidylcholine 22:1, 22:1 (1,2-dierucoyl-sn-glycero-3-phosphocholine) = PC 22:1, 22:1Phosphatidylcholine 22:6, 22:6 (1,2-didocosahexaenoyl-sn-glycero-3-phosphocholine) = PC 22:6, 22:6Docosahexaenoic acid (all-cis-docosa-4,7,10,13,16,19-hexa-enoic acid) = DHA