This dataset contains the dissolved organic matter (DOM) quantification and optical characterisation results from a KOSMOS mesocosm experiment carried out in the framework of the Ocean Artificial Upwelling project. The experiment was carried out in the autumn of 2018 in the oligotrophic waters of Gran Canaria. During the 39 days of experiment nutrient-rich deep water was added to the mesocosms in two modes (singular vs recurring additions), with four levels of intensity. Dissolved organic carbon, nitrogen and phosphorus were quantified with a Shimadzu TOC-5000 and a QuAAtro AutoAnalyzer. The absorption and fluorescence proprieties of DOM were determined making use of an Ocean Optics USB2000+UV-VIS-ES Spectrometer and a Jobin Yvon Horiba Fluoromax-4 spectrofluorometer, respectively. The aim of this dataset was to study the effect of artificial upwelling on the dissolved organic matter pool and its potential implications for carbon sequestration.