Relative abundance of prokaryotes in sediments of the Håkon Mosby mud volcano (Z1-Z3, all habitats)


CARD-FISH was performed as previously described in Ruff et al., (2013; doi:10.1371/journal.pone.0072627) with the following modifications. 4-6 µl of 25-fold diluted sediment were used for filtration. Archaeal cell walls were permeabilized with 0.1M HCl for 2 min to detect ANME-3 cells, or Proteinase K solution (15 µg ml-1 (Merck, Darmstadt, Germany) in 0.05 M EDTA (pH 8), 0.1 M Tris-HCl (pH 8), 0.5 M NaCl) for 2-4 min at room temperature for all other archaea. Bacterial cell walls were permeabilized with lysozyme solution (1000kU/ml) for 60 min at 37°. Cells were stained with DAPI (1µg/ml), embedded in mounting medium and counted in 40-60 independent microscopic fields using an Axiophot II epifluorescence microscope (Carl Zeiss, Jena, Germany).

ANME aggregates had been observed in various samples (#) but they had been NOT included in the relative abundance calculations here.

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Creator Ruff, S Emil ORCID logo; Ramette, Alban ORCID logo; Boetius, Antje ORCID logo
Publisher PANGAEA
Publication Year 2016
Funding Reference Seventh Framework Programme Crossref Funder ID 226354 Hotspot Ecosystem Research and Mans Impact On European Seas; Sixth Framework Programme Crossref Funder ID 36851 European Seafloor Observatory Network
Rights Creative Commons Attribution 3.0 Unported;
OpenAccess true
Resource Type Dataset
Format text/tab-separated-values
Size 290 data points
Discipline Earth System Research
Spatial Coverage (14.702W, 72.000S, 14.748E, 72.007N); North Atlantic; Håkon Mosby Mud Volcano; Norwegian Sea
Temporal Coverage Begin 2003-07-02T00:00:00Z
Temporal Coverage End 2010-10-04T13:01:00Z