The effects on growth performance of supplementation of four different AA combinations in a milk replacer (MR, 25.4% CP and 20.3% fat) based on skimmed milk powder and whey protein concentrate were evaluated in 76 Holstein male calves (3 ± 1.7 d old). The 4 MR were: CTRL with no AA supplementation; PG supplying additional 0.3% Pro and 0.1% Gly; FY supplying additional 0.2% Phe and 0.2% Tyr; KMT providing additional 0.62% Lys, 0.22% Met, and 0.61% Thr. All calves were fed the same milk allowance program and were weaned at 56 d of study. Concentrate intake was limited to minimize interference of potential differences in solid feed intake among treatments. Animals were weighed weekly, intakes recorded daily, and blood samples obtained at 2, 5, and 7 wk of study to determine serum urea and plasma AA concentrations. The file presents data of the backwards selection process to assess the model that fitted better ADG with the study variables (performance, intake, plasma AA balances). Data was collected at Torre Marimon IRTA calf facilities (Caldes de Montbui, Spain) during 2017