Bilayers which float below a self-assembled monolayer (SAM) coated solid support represent one of the most advanced membrane mimetics. Recently (RB 1720155) we assessed the feasibility of depositing floating supported membranes below self-assembled monolayers of (15-Mercaptopentadecyl)-1-aminoethanol (ThiolC15AmideAlcohol). We plan to continue this research by creating eight differing model bilayer systems at the gold/water interface and examining the structures of these biomembrane mimetics using. Each bilayer will be deposited below both pure ThiolC15AmideEthanol SAMs and a mixed 50:50 mixed ThiolC15AmideEthanol and ThiolC15AmideAmine SAMs. We wish to access the relationship between bilayer-SAM electrostatics and water gap distance. From this we aim to determine which sample and SAM conditions are optimal for producing stable floating membranes for a diverse range of lipid species.