Responses of the coastal prokaryotic community to phytoplankton-derived intra- and extra-cellular organic matters

The goal of this study is to demonstrate a hypothesis that phytoplanktonic intracellular organic matters released by cell disruption caused during predation and extracellular organic matters contained in exudate have distinct effects on coastal prokaryotic community. Coastal prokaryotic community collected in Osaka bay was cultured with addition of intracellular or extracellular organic matters extracted from Heterosigma akashiwo (NIES 293 strain), or f divided medium for control. Each triplicated flask was incubated at 20 degree Celsius with a 10 h 14 h light photocycle. Samples were taken on the day 1, 3, 5, 7, 9, 11, and 13 during incubation. DNA extracted from each samples were offered for 16S rRNA (V3 V4 region) analysis.

Identifier
Source https://data.blue-cloud.org/search-details?step=~0120CF955042BD6550548DBE0280D5C8396D198B095
Metadata Access https://data.blue-cloud.org/api/collections/0CF955042BD6550548DBE0280D5C8396D198B095
Provenance
Instrument Illumina MiSeq; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Contributor Laboratory of Marine Microbiology, Graduate School of Agriculture, Kyoto University
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Spatial Coverage (135.100W, 34.300S, 135.100E, 34.300N)
Temporal Coverage Begin 2017-11-07T00:00:00Z
Temporal Coverage End 2017-11-21T00:00:00Z