Interfacial adsorption of monoclonal antibody and antigen binding

DOI

Antibody-antigen binding has been extensively used in many technological applications such as immunoassays, chemical analysis and bionanotechnology. The crucial element underlying these processes is the high molecular specificity. The binding of antigen to surface immobilized antibody is widely used to detect the antibody¿s bioactivity. Changes in the bioactivity reveal in situ structural conformation as well as interfacial packing density. The aim of this study is to determine antibody layer thickness and packing density by neutron reflection. Neutron reflection study is an important step towards the establishment of the relation between antigen binding capacity and the structure of the interfacially immobilized antibody.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24079926
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24079926
Provenance
Creator Professor Jian Lu; Ms Donghui Jia; Miss Maria Rodriguez-Rius; Dr Devashi Adroja
Publisher ISIS Neutron and Muon Source
Publication Year 2013
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2010-02-25T08:45:17Z
Temporal Coverage End 2010-05-10T07:45:54Z