Synthetic syntrophy for adenine nucleotide cross-feeding between metabolically active nanoreactors

DOI

Living systems depend on continuous energy input for growth, replication, and information processing. Cells convert light or chemical energy of nutrients into other forms of energy, such as ion gradients or adenosine triphosphate (ATP). However, engineering sustained fueling pathways in synthetic systems is challenging. Inspired by endosymbionts that rely on the host cell for their nutrients, we introduce the concept of cross-feeding between synthetic submicron-size vesicles that can exchange ATP and ADP across their membranes. One population of vesicles produces and exports ATP, while a second population of vesicles takes up ATP to fuel energy-consuming reactions. The produced ADP feeds back to the first vesicles. The vesicles are a platform technology to fuel ATP-dependent processes in a sustained fashion, with potential applications in synthetic cells and nanoreactors. Fundamentally, the vesicles enable studying non-equilibrium processes in an energy-controlled environment and promote the development and understanding of constructing life-like metabolic systems.

Identifier
DOI https://doi.org/10.34894/YPORKS
Related Identifier IsCitedBy https://doi.org/10.1038/s41565-024-01811-1
Metadata Access https://dataverse.nl/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.34894/YPORKS
Provenance
Creator Laura Heinen ORCID logo; Marco van den Noort (ORCID: 0000-0002-2025-461X); Martin S. King ORCID logo; Edmund R.S. Kunji ORCID logo; Bert Poolman (ORCID: 0000-0002-1455-531X)
Publisher DataverseNL
Contributor Groningen Digital Competence Centre; DataverseNL network
Publication Year 2024
Funding Reference ERC Proof-of-Concept grant FuelVesicles – 899477 ; NWO Gravitation program Building-a-Synthetic-Cell BaSyC – TNW 17.389 ; European Union's Horizon 2020 research and innovation programme, oLife MSCA-COFUND 847675 ; UK Medical Research Council MC_UU_00028/2
Rights CC0 1.0; info:eu-repo/semantics/openAccess; http://creativecommons.org/publicdomain/zero/1.0
OpenAccess true
Contact Groningen Digital Competence Centre (Groningen University)
Representation
Resource Type Dataset
Format application/vnd.chemdraw+xml; image/tiff; image/jpeg; application/vnd.openxmlformats-officedocument.spreadsheetml.sheet; text/plain
Size 61419; 3145850; 117118; 86741; 16530; 144474; 63714; 13275; 754530; 10975; 3906
Version 1.0
Discipline Biology; Life Sciences; Medicine